±Ý¾×Àº (TA cloning vector½Ã) cloning ºñ¿ë
176,000 (cloning)+120000 (sequencing)+primerÁ¦ÀÛºñ¿ë ÀÌ·¸°Ô µé¾î°£´Ù´Â ¸»¾¸À̽Ű¡¿ä?
±×·¸´Ù¸é Á¦°¡ ÁغñÇØ¾ß ÇÒ °ÍÀÌ sample cDNA¿Í primerÀΰ¡¿ä?
ÀÌ°ÍÀº ¾î¶»°Ô µå·Á¾ß ÇÒ±î¿ä?
Á¦³ë¸¶ÀÎ ´ÔÀÇ ¿øº»±Û
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¾È³çÇϼ¼¿ä °ÇöÁÖ ¼±»ý´Ô
cloning °¡°ÝÀº Çѹø °¡°ÝÀº 176,000¿ø ÀÔ´Ï´Ù.
±×¸®°í VECTOR PCDNA3.1(+)-MYC-HIS¿¡ ¹Ù·Î ³Ö¾î µå¸®Áö´Â ¸øÇÏ°í, T-vector cloningÀ» ÇÑ µÚ VECTOR PCDNA3.1(+)-MYC-HIS¿¡ cloning ¿¡ µé¾î °¡¾ß ÇÒ°Í °°½À´Ï´Ù. ±×·³ ÃÑ µÎ¹øÀÇ cloning stepÀÌ µé¾î °¡°Ô µË´Ï´Ù.
sequencingÀº ÀúÈñ°¡ ÀÇ·Ú Çϴ°÷¿¡¼ 700bpÁ¤µµ Àб⠶§¹®¿¡, Á¤È®ÇÏ°Ô 2.7kb¸ðµÎ È®ÀÎ ÇÒ·Á¸é 6¹øÀÇ sequencing ÀÌ µé¾î°¡°Ô µÇ°í, sequencing Çϴµ¥ »õ·Î µðÀÚÀÎ ÇؾßÇÒ primer°¡ 4°³ ÇÊ¿ä ÇÒ°Í °°½À´Ï´Ù.
±×¸®°í Å« size DNA ÀÇ °æ¿ì PCR µµÁßpoint mutation ÀÌ ÀÏ¾î ³¯ °¡´É¼ºÀÌ Àֱ⶧¹®¿¡ sequencing ÇÒ sampleÀº 2°³¸¦ º¸³À´Ï´Ù.
±×·¯¸é, ÃÑ sequencing°¹¼ö´Â 12°³°¡ ÀÇ·Ú µË´Ï´Ù.
sequencing °¡°ÝÀº Çѹø¿¡ 10,000¿ø ºÎ°¡ µË´Ï´Ù.
±×¸®°í ÃÑ ¿¹»ó ±â°£Àº 7ÁÖÁ¤µµ ¼Ò¿äµÉ°Í °°½À´Ï´Ù.
°ÇöÁÖ ´ÔÀÇ ¿øº»±Û
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mRNA Sequence : NM_000222
NAME: KIT (v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog. Homo sapiens) À¯ÀüÀÚ¸¦ cloning (¾à 2.7KB)ÇÏ°í ½Í½À´Ï´Ù. cDNA ¹× PRIMER¸¦ Á¦°¡ °¡Áö°í ÀÖ½À´Ï´Ù. ÀÌ·²°æ¿ì °¡°ÝÀÌ ¾ó¸¶³ª µÇ´ÂÁö ¾Ë ¼ö ÀÖÀ»±î¿ä? VECTOR PCDNA3.1(+)-MYC-HISÀÔ´Ï´Ù. ÀúÈñ°¡ Á¦°øÇص帱¼ö ÀÖÀ¸´Ï ¿©±â¿¡´Ù CLONINGÇÏ°í ½Í½À´Ï´Ù. SEQUENCING ºñ¿ëÀº Çѹø¿¡ ¾ó¸¶Âë Çϳª¿ä?? TOTAL SEQUENCING Á¤º¸µµ Á¦°øÇØ Áֽóª¿ä?? ¶ÇÇÑ È¨ÆäÀÌÁö¿¡ º¸´Ï 1°³¿ùÁ¤µµ °É¸®´Â °Í °°Àºµ¥, ÀúÈñ°¡ ÀÇ·ÚÇÏ´Â Å©±âÀÇ À¯ÀüÀÚ¸¦ Ŭ·Î´×Çؼ ¿Ï·áÇϴµ¥ º¸Åë ¾î´ÀÁ¤µµ °É¸®½Ã³ª¿ä?
ºü¸¥ ´äº¯ ºÎŹµå¸³´Ï´Ù.
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