GENOMINE

2-D Electrophoresis 2DE plus Protein Identification Package

2-D Electrophoresis provides two dimensional gel electrophoresis custom services.

Step1. 1st dimensional IEF

24cm length and non-linear IPG strip
Broad range(pH4-10), 2unit or 3unit ranges
(pH4.5-6.5 / pH4-7 / pH6-8 / pH6-9)

Step2. 2nd dimensional SDS-PAGE

24cm x 20cm x 1mm, 10-16% gradient gel

Step3. Staining

MALDI-compatible ammoniacal Silver staining
(Detection limit 0.2ng/spot)
Colloidal Coomassie G-250 staining (Detection limit 1ng/spot)
SYPRO RUBY staining (Detection limit 1ng/spot)

Step4. Image Analysis

PDQuest Image Analysis Program

Step5. Statistical Analysis

Distance tree Analysis/Clustering Analysis

2D MAP

Arabidopsis seedling2D map

Arabidopsis seedling2D map protein information service

Genomine Proteomics Group에서 구축한 Arabidopsis thaliana seedling 2D map D/B의 단백질 동정정보를 image matching을 통하여 확인할 수
있습니다. 현재 Arabidopsis thaliana seedling 2D map D/B는 지속적으로
update 되고 있으며, 향후에는 MS analysis에 우선하여 image matching으로 단백질 발현정보와 함께 동정된 단백질을 확인할 수 있는 완벽한 protein information D/B 구축을 목표로 하고 있습니다.

대상: Arabidopsis seedling samples 2DE service 의뢰 고객

plasma Albumin depleted 2D map

Albumin free vitamin K dependent protein enriched plasma fraction

Vitamin K dependent( in protein systhesis) blood coagulation factor 및 그들의 isomer, processed derivatives를 확인 할 수 있는 plasma fraction.
그 외 whole plasma map에서 확인할 수 없는 protein spot(red cross hair)이 포함되어 있습니다.

Sample image

Service Details

서비스항목 구성된 표
Cat. No.	Service Name	Format	Price(￦)	Duration
D3010	Silver Stained 2-DE Ge Plus Spot Quantification	B	300,000	7 days
D3011	Coomassie Blue Stained 2-DE Gel Plus Spot Quantification		280,000	7 days
D3012	Sypro Ruby Stained 2-DE Gel Plus Spot Quantification		360,000	7 days
D3013	Silver Stained 2-DE Ge Plus Expression Pattern Classification	C	350,000	7 days
D3014	Coomassie Blue Stained 2-DE Gel Plus Expression Pattern Classification		320,000	7 days
D3015	Sypro Ruby Stained 2-DE Gel Plus Expression Pattern Classification		400,000	7 days

*Duration: working day

For sample preparation and information on service procedures
Contact : gjkim@genomine.com

Step1. 1st dimensional IEF

24cm length and non-linear IPG strip
Broad range(pH4-10), 2unit or 3unit ranges (pH4.5-6.5 / pH4-7 / pH6-8 / pH6-9)

Step2. 2nd dimensional SDS-PAGE

24cm x 20cm x 1mm, 10-16% gradient gel

Step3. Staining

MALDI-compatible ammoniacal Silver staining (Detection limit 0.2ng/spot)
Colloidal Coomassie G-250 staining (Detection limit 1ng/spot)
SYPRO RUBY staining (Detection limit 1ng/spot)

Step4. Image Analysis

PDQuest Image Analysis Program

Step5.Statistical Analysis

Distance tree Analysis/Clustering Analysis

Service Package Pricing

서비스패키지항목 구성된 표
	A format	B format	Price(￦)
Package 1	2DE 1-4 gels	2DE 1-4 gels	5,000,000
	All spot selection	All spot selection
	PMF identification 66ea	MALDI-TOF/TOF 35ea
Package 2	2DE 1-4 gels	2DE 1-4 gels	7,000,000
	All spot selection	All spot selection
	PMF identification v	MALDI-TOF/TOF 50ea
Package 3	2DE 1-4 gels	2DE 1-4 gels	10,000,000
	All spot selection	All spot selection
	PMF identification 150ea	MALDI-TOF/TOF 75ea

Service Details

서비스항목 구성된 표
Cat. No.	Service Name	Price(￦)	Duration
P1010	Package1 Aformat 2DE plus PMF	5,000,000	-
P1020	Package1 B format 2DE plus TOF/TOF	5,000,000	-
P2010	Package2 A format 2DE plus PMF	7,000,000	-
P2020	Package2 B format 2DE plus TOF/TOF	7,000,000	-
P3010	Package3 A format 2DE plus PMF	10,000,000	-
P3020	Package3 B format 2DE plus TOF/TOF	10,000,000	-

SERVICES

SERVICES

2-D Electrophoresis

Arabidopsis seedling2D map protein information service

Albumin free vitamin K dependent protein enriched plasma fraction

Service Details

Service Package Pricing

Service Details