Phosphoprotein Analysis provides phosphoprotein specific enrichment from bio-material, one- or two-dimensional gel electrophoresis, phosphoprotein staining and image analysis custom services.
- PhosPro is proprietary technology of GENOMINE for phosphoprotein enrichment
STEP1. Phosphoprotein enrichment
Phos-Pro™ kit(Genomine)
STEP2. 1st dimensional IEF
12cm length and non-linear IPG strip
Broad range(pH4-10), 2unit or 3unit ranges
(pH4.5-6.5 / pH4-7 / pH6-8 / pH6-9)
STEP3. 2nd dimensional SDS-PAGE
12cm x 10cm x 1mm/ 10-16% gradient gel
STEP4. Multiple Staining
Pro-Q™ Diamond phosphoprotein staining (Detection limit 1ng/spot)
Colloidal Coomassie G-250 staining (Detection limit 1ng/spot)
STEP5. Image Analysis
Differential display & Comparative analysis
STEP6. Mass Analysis
Phosphoprotein or Phosphopeptide Site Determination
Example of PhosPro Technology for phosphoprotein enrichment
Service Details
| Cat. No. | Service Name | Price(￦) | Duration |
|---|---|---|---|
| P8010 | Phosphoprotein staining service (SDS-PAGE) | 300,000 | 7 days |
| P8011 | Phosphoprotein staining service (2-DE Gel) | 360,000 | 7 days |
| P8020 | Phosphoprotein identification by PMF | 100,000 | 7 days |
| P8030 | Phosphopeptide identification by PSD | 100,000 | 7 days |
| P8040 | Phosphorylation site determination by CAF sequencing | 100,000 | 7 days |
| P8050 | Phosphoprotein enrichment plus Service item1 | 360,000 | 7 days |
| P8060 | Phosphoprotein identification by LC-MS/MS | 280,000 | 7 days |
*Duration: working day
- For sample preparation and information on service procedures
- Contact : gjkim@genomine.com